ADD:
This is true even though the the heteroduplexes
formed in this case typically have distinct melting
behavior. 

Finally, we model and optimize the
separation of heteroduplex contents of the three
genotypes in terms of mixture proportion.

\bigskip

ADD?

We begin by asking what types of duplexes 
are present for melting and in what proportions, when we 
mix wild-type and homozygous mutant DNA in proportion
$x$ (wild-type) and $1-x$ (homozygous mutant), melt
then quickly decrease the temperature so that complementary and
nearly complementary strands independently, i.e.,  solely in
proportion to their concentrations.


ADD?
Just observe the het case to wt = wt + plus half het, hom=half het

ADD?

Use f instead of g or say they are interchangeable

Note that when x=0, unspiked:

WT = f1 (or g1)

MUT = f4 (or g4)

HET = 1/4 f1 + 1/4 f2 + 1/4 f3 + 1/4 f4

and note that fs take into account that different duplexed might have
different intensities and the derivation still holds as long as
f1=f4.  f2 doesn't have to equal f3 and can have different proportionality
to their concentrations than f1=f4
