Optimal spiking with a wild-type homozygote to maximize heteroduplex concentration separation among genotypes for SNP genotyping with high-resolution melting curves.

A graph indicating the concentration of heteroduplexes for the three types of sample as a function of the proportion of spike (to the total spike plus unknown) is here.

The values visualized by these animations correspond to the left edge of the graph where x=0, and the vertical blue line where x=1/(1+6)=1/7.